产品信息
| 名称 | SK-N-DZ (人神经母细胞瘤细胞) (STR鉴定正确) |
| 别称 | SKNDZ |
| 种属 | 人 |
| 生长特性 | 贴壁细胞 |
| 细胞形态 | 成神经细胞样 |
| 冻存条件 | 冻存液：55% 基础培养基+40%FBS+5%DMSO 温度：液氮 |
| 培养方案A（默认） |
培养条件:
气相：空气，95%；CO2，5%, 温度：37℃
|
| 注意事项 | 该细胞贴壁松散，操作时请尽量轻柔；换液时需预热培养基；收货如有大块脱落或全部脱落的细胞团，为正常现象，请按照收货注意事项处理；细胞离心复苏之后有少量漂浮，第二天换液即可，每三天换液一次，等细胞密度达到80%左右，按照1:2进行传代，传代之后有少量漂浮，换液即可，消化时间在显微镜下观察，以细胞变圆，脱落为主，大约控制消化时间在4-6mim左右。 |
| 推荐传代比例 | 1:3-1:4 |
| 推荐换液频率 | 2-3次/周 |
| 注意事项 | 该细胞贴壁松散，操作时请尽量轻柔；换液时需预热培养基；收货如有大块脱落或全部脱落的细胞团，为正常现象，请按照收货注意事项处理；细胞离心复苏之后有少量漂浮，第二天换液即可，每三天换液一次，等细胞密度达到80%左右，按照1:2进行传代，传代之后有少量漂浮，换液即可，消化时间在显微镜下观察，以细胞变圆，脱落为主，大约控制消化时间在4-6mim左右。 |
参考资料(来源文献)
| 背景描述 | SK-N-DZ are neuroblasts that were isolated from the brain of a 2-year-old, White female patient with neuroblastoma.SK-N-DZ are neuroblasts that were isolated from the brain of a 2-year-old, White female patient with neuroblastoma. Expression of the N-myc gene product was reduced in differentiated SK-N-DZ cells as compared with undifferentiated cells. Expression of the c-src gene product, pp60c-src was enhanced in differentiated SK-N-DZ cells as was tyrosine phosphorylation of cellular proteins. The cells exhibit moderate MDR1 expression. Retinoic acid induces differentiation in this line. |
| 年龄（性别） | 女性；2岁 |
| 组织来源 | 大脑 |
| 细胞类型 | 肿瘤细胞 |
| 肿瘤类型 | 神经母细胞瘤细胞 |
| 生物安全等级 | BSL-1 |
| 致瘤性 | Yes; Yes, forms tumors in nude mice |
| 保藏机构 | ATCC; CRL-2149 |
