产品信息
| 名称 | hCMEC/D3 (人脑微血管内皮细胞) (STR鉴定正确) |
| 别称 | HCMEC/D3; CMEC/D3; human Cortical Microvessels Endothelial Cells/D3 |
| 种属 | 人类 |
| 生长特性 | 贴壁细胞 |
| 细胞形态 | 纺锤形细胞 |
| 冻存条件 | 冻存液：55% 基础培养基+40% FBS+5% DMSO 温度：液氮 |
| 培养方案A（默认） |
生长培养基:
培养条件:
气相：空气，95%；CO2，5%, 温度：37℃
|
| 推荐传代比例 | 1:3-1:6 |
| 推荐换液频率 | 2-3次/周 |
参考资料(来源文献)
| 背景描述 | The hCMEC/D3 cell line was derived from human temporal lobe microvessels isolated from tissue excised during surgery for control of epilepsy. The primary isolate was enriched in cerebral endothelial cells (CECs). In the first passage, cells were sequentially immortalized by lentiviral vector transduction with the catalytic subunit of human telomerase (hTERT) and SV40 large T antigen, following which CEC were selectively isolated by limited dilution cloning, and clones were extensively characterized for brain endothelial phenotype. This brain microvascular endothelial cell line represents one such model of the human BBB that can be easily grown and is amenable to cellular and molecular studies on pathological and drug transport mechanisms with relevance to the central nervous system (CNS). |
| 年龄（性别） | 女性；成人 |
| 组织来源 | 脑微血管 |
| 细胞类型 | 转化细胞系 |
| 生物安全等级 | BSL-2 |
| 保藏机构 | KCB; KCB 2019022YJ |
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