产品信息
| 名称 | SU-DHL-1 (人间变性大细胞淋巴瘤细胞) (STR鉴定正确) |
| 别称 | SU-DHL1; SUDHL1; SUDHL-1; SuDHL-1; SuDHL 1; Stanford University-Diffuse Histiocytic Lymphoma-1 |
| 种属 | 人源 |
| 生长特性 | 悬浮细胞 |
| 细胞形态 | 淋巴母细胞样 |
| 冻存条件 | 冻存液：55% 基础培养基+40%FBS+5%DMSO 温度：液氮 |
| 培养方案A（默认） |
培养条件:
气相：空气，95%；CO2，5%, 温度：37℃
|
| 推荐传代比例 | 1:2-1:6 |
| 推荐换液频率 | 2-3次/周 |
参考资料(来源文献)
| 背景描述 | SU-DHL-1 is a histiocytic cell that was isolated from the lymph node of a White, 10-year-old, male patient with large cell lymphoma. The cells are surface Ig negative (sIG-). The cells are non-specific esterase, acid phosphatase and oil red O positive. The cells are periodic acid Schiff negative. These cells phagocytose Candida albicans and latex particles. The cells are reported to be very weakly E - rosette positive. ATCC confirmed this cell line is negative for the presence of Epstein-Barr viral DNA sequences via PCR. |
| 年龄（性别） | 男性；10岁 |
| 组织来源 | 淋巴结 |
| 细胞类型 | 肿瘤细胞 |
| 肿瘤类型 | 淋巴瘤细胞 |
| 生物安全等级 | BSL-1 |
| 倍增时间 | ~40-50 hours (DSMZ=ACC-356) |
| 受体表达情况 | CFS-1, expressed; (IL-1-R; IL-2-Rα; IL-6-R; TNFα-R; & c-fms), expressed; Surface Receptors: (Fc; IgMEAC; IgGEA; & E), not expressed |
| 抗原表达情况 | Monocyte Marker: CD163+ Lymphoid Marker: CD45- Progenitor Markers: CD10-, CD34- Activation Markers: CD30+, CD25+, CD70+, CD71+, CD80-, HLA-DR+, CD45- T-Cell Markers: CD2-, CD3-, CD4-, CD5+, CD7-, CD8- B-Cell Markers: CD19-, CD20-, CD21-, CD22- Myelom |
| 基因表达情况 | fusion gene NPM-ALK (p80)+; anaplastic lymphoma kinase (ALK)+; Ig not expressed |
| 保藏机构 | ATCC; CRL-2955 |
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