产品信息
| 别名 | Endogenous Mediator (LEM), Mononuclear Cell Factor (MCF) |
| 物种 | Human |
| 表达宿主 | E.coli |
| 序列信息 | MQAKHKQRKRLKSSCKRHPLYVDFSDVGWNDWIVAPPGYHAFYCHGECPFPLADHLNSTNHAIVQTLVNSVNSKIPKACCVPTELSAISMLYLDENEKVVLKNYQDMVVEGCGCR with polyhistidine tag at the C-terminus |
| 检索号 | P12643 |
| 分子量 | 13.76 kDa |
| 表观分子量 | 14 kDa |
| 标签 | His-tag at the C-terminus |
| 生物活性 | Measure by its ability to induce alkaline phosphatase production by ATDC5 cells.The ED50 for this effect is <9.5 ng/mL.The specific activity of recombinant BMP-2 is > 3.2 x 10^6IU/mg. |
产品特性
| 纯度 | >95% as determined by SDS-PAGE. Ni-NTA chromatography. |
| 内毒素 | <0.1 EU per 1 μg of the protein by the LAL method. |
| 保存 | Lyophilized protein should be stored at -5~-20°C for 1 year. Upon reconstitution, store at 2-8°C for up to 1 week. Further dilute in a buffer containing a carrier protein or stabilizer (e.g.0.1%BSA,10%FBS,5%HSA or 5% trehalose solution), protein aliquots should be stored at -5~-20°C or -80°C for 3-6 months. |
| 运输 | Ambient temperature or ice pack. |
| 制剂 | The protein was lyophilized from a 0.2 µm filtered solution containing 20 mM sodium citrate, 0.2 M NaCl, pH 3.5. |
| 复融 | It is recommended to reconstitute the lyophilized protein in sterile water to a concentration not less than 100 μg/mL.Do Not Vortex! Vigorous shaking may impair the biological activity of the protein. |
背景介绍
BMP-2 like other bone morphogenetic proteins, plays an important role in the development of bone and cartilage. It is involved in the hedgehog pathway, TGF beta signaling pathway, and in cytokine-cytokine receptor interaction. It is also involved in cardiac cell differentiation and epithelial to mesenchymal transition. Like many other proteins from the BMP family, BMP-2 has been demonstrated to potently induce osteoblast differentiation in a variety of cell types. BMP-2 may be involved in white adipogenesis and may have metabolic effects.
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