产品信息
别名 | TGF-BETA-1, TGFB1 |
物种 | Swine |
表达宿主 | E.coli |
序列信息 | MALDTNYCFSSTEKNCCVRQLYIDFRKDLGWKWIHEPKGYHANFCLGPCPYIWSLDTQYSKVLALYNQHNPGASAAPCCVPQALEPLPIVYYVGRKPKVEQLSNMIVRSCKCS with polyhistidine tag at the C-terminus. |
检索号 | P07200.2 |
分子量 | 13.7 kDa |
表观分子量 | 11-17 kDa |
标签 | His-tag at the C-terminus |
生物活性 | Measure by its ability to inhibit IL-4-induce proliferation in HT-2 cells.The ED50 for this effect is <0.1 ng/mL. |
产品特性
纯度 | >98% as determined by SDS-PAGE. Ni-NTA chromatography |
内毒素 | <0.1 EU per 1 μg of the protein by the LAL method. |
保存 | Lyophilized protein should be stored at -5~-20°C for 1 year. Upon reconstitution, store at 2-8°C for up to 1 week. Further dilute in a buffer containing a carrier protein or stabilizer (e.g.0.1%BSA,10%FBS,5%HSA or 5% trehalose solution), protein aliquots should be stored at -5~-20°C or -80°C for 3-6 months. |
运输 | Ambient temperature or ice pack. |
制剂 | The protein was lyophilized from a 0.2 µm filtered solution containing 20 mM sodium citrate, 0.2 M NaCl, pH 4.5. |
复融 | It is recommended to reconstitute the lyophilized protein in sterile water to a concentration not less than 100 μg/mL.Do Not Vortex! Vigorous shaking may impair the biological activity of the protein.In some experiments, it recommends to add 10 mM HCl when reconstitute lyophilized protein. |
背景介绍
Transforming growth factor beta 1 or TGF-β1 is a polypeptide member of the transforming growth factor beta superfamily of cytokines. It is a secreted protein that performs many cellular functions, including the control of cell growth, cell proliferation, cell differentiation, and apoptosis. In humans, TGF-β1 is encoded by the TGFB1 gene.
FAQs
Q:{{item.question}}
A:
暂无数据