产品信息
| 别名 | Pro-neuregulin-1, Neuregulin-1 beta 1, NRG1-beta 1, HRG1-beta 1, EGF, NRG1, GGF, HGL, HRGA, NDF, SMDF, Heregulin-β1 |
| 物种 | Human |
| 表达宿主 | E.coli |
| 序列信息 | A DNA sequence encoding Human Heregulin Beta 1 Protein (#Q02297-6) (Ser177-Glu241) was expressed with polyhistidine tag at the C-terminus. |
| 检索号 | Q02297-6 |
| 分子量 | 8.42 kDa |
| 表观分子量 | 8 kDa |
| 标签 | His Tag (C-term) |
| 生物活性 | Measure by its ability to induce MCF-7 cells proliferation. The ED₅₀ for this effect is < 10 ng/mL. |
产品特性
| 纯度 | >95% as determined by SDS-PAGE. Ni-NTA chromatography. |
| 内毒素 | <0.1 EU per 1 μg of the protein by the LAL method. |
| 保存 | Lyophilized protein should be stored at -5~-20°C for 1 year. Upon reconstitution, store at 2-8°C for up to 1 week. Further dilute in a buffer containing a carrier protein or stabilizer (e.g.0.1% BSA, 10% FBS, 5% HSA or 5% trehalose solution), protein aliquots should be stored at -5~-20°C or -80°C for 3-6 months. |
| 运输 | Ambient temperature or ice pack. |
| 制剂 | The protein was lyophilized from a 0.2 µm filtered solution containing 1 × PBS, pH8.0. |
| 复融 | It is recommended to reconstitute the lyophilized protein in sterile water to aconcentration not less than 100 μg/mL. Do Not Vortex! Vigorous shaking may impair the biological activity of the protein. |
背景介绍
Neuregulin-1 (NRG-1, also called heuregulin1 or neu differentiation factor) is a glycoprotein that belongs to the neuregulins family. Structurally, Neuregulin-1 harbors tissue-specific N terminal sequence, followed by immunoglobulin-like (Ig-like) domains, an EGF-like domain, a transmembrane domain, and a cytoplasmic domain. NRG1 has multiple isoforms produced by alternative splicing. Heregulin-β1 (HRG-β1) is one of the isoforms, has been reported to engage the development and survival of cardiomyocytes derived from embryonic stem (ES) cells via activating MAPK-ERK and PI3K-AKT pathways. Moreover, HRG-β1 plays a central role in promoting the proliferation of neuronal progenitors from embryonic neural stem cells.
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