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IGF-I/Somatamedin C/IGF-IA,Mouse,Recombinant

货号:PCK018

价格:¥500¥780¥58808280

规格:
10μg
  • 10μg
  • 50μg
  • 500μg
  • 1mg
数量: - +

产品信息

别名 Somatamedin C, IGF-IA
物种 Mouse
表达宿主 E.coli
序列信息 MGPETLCGAELVDALQFVCGPRGFYFNKPTGYGSSIRRAPQTGIVDECCFRSCDLRRLEMYCAPLKPTKAA with polyhistidine tag at the C-terminus.
检索号 P05017.2
分子量 8.61 kDa
表观分子量 11-17 kDa
标签 His-tag at the C-terminus
生物活性 Measure by its ability to induce MCF-7 cells proliferation. The ED50 for this effect is <2 ng/mL. The specific activity of recombinant mouse IGF-I is > 5 x 10^5 IU/mg.

产品特性

纯度 >98% as determined by SDS-PAGE. Ni-NTA chromatography
内毒素 <0.1 EU per 1 μg of the protein by the LAL method.
保存 Lyophilized protein should be stored at -5~-20°C for 1 year. Upon reconstitution, store at 2°C to 8°C for up to 1 week. Further dilute in a buffer containing a carrier protein or stabilizer (e.g. 0.1% BSA, 10%FBS, 5%HSA or 5% trehalose solution), protein aliquots should be stored at -5~-20°C or -80°C for 3-6 months.
运输 Ambient temperature or ice pack.
制剂 The protein was lyophilized from a 0.2 µm filtered solution containing 1X PBS, pH 8.0.
复融 It is recommended to reconstitute the lyophilized protein in sterile water to a concentration not less than 100 μg/mL.Do Not Vortex! Vigorous shaking may impair the biological activity of the protein.

背景介绍

Insulin-like growth factor 1 (IGF-1), also called somatomedin C, is a protein that in humans is encoded by the IGF1 gene. IGF-1 is a hormone similar in molecular structure to insulin. It plays an important role in childhood growth and continues to have anabolic effects in adults. A synthetic analog of IGF-1, mecasermin, is used for the treatment of growth failure.
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