本司产品仅用于科研,不用于临床诊断和治疗

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G-CSF/CSF-3/MGI-1G, Human, Recombinant

货号:PCK040

价格:¥1780¥4980¥1388019980

规格:
10μg
  • 10μg
  • 50μg
  • 500μg
  • 1mg
数量: - +

产品信息

别名 CSF-3, MGI-1G, GM-CSF beta, pluripoietin
物种 Human
表达宿主 E.coli
序列信息 TPLGPASSLPQSFLLKCLEQVRKIQGDGAALQEKLCATYKLCHPEELVLLGHSLGIPWAPLSSCPSQALQLAGCLSQLHSGLFLYQGLLQALEGISPELGPTLDTLQLDVADFATTIWQQMEELGMAPALQPTQGAMPAFASAFQRRAGGVLVASHLQSFLEVSYRVLRHLAQP with polyhistidine tag at the N-terminus.
检索号 P09919.1
分子量 19.48 kDa
表观分子量 21 kDa
标签 His-tag at the N-terminus
生物活性 Measure by its ability to induce proliferation in NFS-60 cells. The ED50 for this effect is <50 pg/mL. The specific activity of recombinant human G-CSF is > 2 x 10^7 IU/mg.

产品特性

纯度 >98% as determined by SDS-PAGE. Ni-NTA chromatography
内毒素 <0.1 EU per 1 μg of the protein by the LAL method.
保存 Lyophilized protein should be stored at -5~-20°C for 1 year. Upon reconstitution,store at 2-8°C for up to 1 week. Further dilute in a buffer containing a carrier protein or stabilizer (e.g.0.1% BSA,10%FBS,5%HSA or 5% trehalose solution), protein aliquots should be stored at -5~-20°C or -80°C for 3-6 months.
运输 Ambient temperature or ice pack.
制剂 The protein was lyophilized from a 0.2 µm filtered solution containing 1X PBS, pH 7.4.
复融 It is recommended to reconstitute the lyophilized protein in sterile water to a concentration not less than 100 μg/mL.Do Not Vortex! Vigorous shaking may impair the biological activity of the protein.

背景介绍

G-CSF is a hematopoietic growth factor. It can activate the progress that committed progenitor cells develop to neutrophils and enhance the functional activities of the mature end-cell. It is secreted in response to specific stimulation by a variety of cells, including bone marrow stroma, macrophages, endothelial cells and fibroblasts. In clinical treatment, G-CSF is used to facilitate hematopoietic recovery after bone marrow transplantation.
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