产品信息
| 别名 | CSF-3, MGI-1G, GM-CSF beta, pluripoietin |
| 物种 | Human |
| 表达宿主 | E.coli |
| 序列信息 | TPLGPASSLPQSFLLKCLEQVRKIQGDGAALQEKLCATYKLCHPEELVLLGHSLGIPWAPLSSCPSQALQLAGCLSQLHSGLFLYQGLLQALEGISPELGPTLDTLQLDVADFATTIWQQMEELGMAPALQPTQGAMPAFASAFQRRAGGVLVASHLQSFLEVSYRVLRHLAQP with polyhistidine tag at the N-terminus. |
| 检索号 | P09919.1 |
| 分子量 | 19.48 kDa |
| 表观分子量 | 21 kDa |
| 标签 | His-tag at the N-terminus |
| 生物活性 | Measure by its ability to induce proliferation in NFS-60 cells. The ED50 for this effect is <50 pg/mL. The specific activity of recombinant human G-CSF is > 2 x 10^7 IU/mg. |
产品特性
| 纯度 | >98% as determined by SDS-PAGE. Ni-NTA chromatography |
| 内毒素 | <0.1 EU per 1 μg of the protein by the LAL method. |
| 保存 | Lyophilized protein should be stored at -5~-20°C for 1 year. Upon reconstitution,store at 2-8°C for up to 1 week. Further dilute in a buffer containing a carrier protein or stabilizer (e.g.0.1% BSA,10%FBS,5%HSA or 5% trehalose solution), protein aliquots should be stored at -5~-20°C or -80°C for 3-6 months. |
| 运输 | Ambient temperature or ice pack. |
| 制剂 | The protein was lyophilized from a 0.2 µm filtered solution containing 1X PBS, pH 7.4. |
| 复融 | It is recommended to reconstitute the lyophilized protein in sterile water to a concentration not less than 100 μg/mL.Do Not Vortex! Vigorous shaking may impair the biological activity of the protein. |
背景介绍
G-CSF is a hematopoietic growth factor. It can activate the progress that committed progenitor cells develop to neutrophils and enhance the functional activities of the mature end-cell. It is secreted in response to specific stimulation by a variety of cells, including bone marrow stroma, macrophages, endothelial cells and fibroblasts. In clinical treatment, G-CSF is used to facilitate hematopoietic recovery after bone marrow transplantation.
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