产品信息
| 别名 | Interferon Alpha-2; IFN-Alpha-2; Interferon Alpha-A; LeIF A; IFNA2 |
| 物种 | Human |
| 表达宿主 | E.coli |
| 序列信息 | Cys24-Glu188 |
| 检索号 | P01563 |
| 分子量 | 19.4 kDa |
| 表观分子量 | 17 kDa |
| 生物活性 | Measured in antiviral assay using A549 human lung cancer cells infected with vesicular stomatitisvirus (VSV). The ED50 for this effect is 5 ng/mL. |
产品特性
| 纯度 | >95% as determined by reducing SDS-PAGE. |
| 内毒素 | <1.0 EU per µg as determined by LAL test. |
| 保存 | Lyophilized protein should be stored at -5~-20℃, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at -5~-20℃ for 3 months. |
| 运输 | Ambient temperature or ice pack. |
| 制剂 | Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.2. |
| 复融 | Always centrifuge tubes before opening. Do not mix by vortex or pipetting.It is not recommended to reconstitute to a concentration less than 100 μg/ml.Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
背景介绍
At least 23 different variants of IFN-α are known. The individual Proteins have molecular masses between 19-26 kDa and consist of Proteins with lengths of 156-166 and 172 amino acids. All IFN-α subtypes possess a common conserved sequence region between amino acid positions 115-151 while the amino-terminal ends are variable. Many IFN-α subtypes differ in their sequences by only one or two positions. Naturally occurring variants also include Proteins that are truncated by 10 amino acids at the carboxyl-terminal end.
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