产品信息
| 别名 | SGRF; IL-23p19; CLMF p40; IL-12 subunit p40; NKSF2 |
| 物种 | Human |
| 表达宿主 | Human Cells |
| 序列信息 | Arg20-Pro189&Ile23-Ser328 |
| 检索号 | Q9NPF7&P29460 |
| 分子量 | 19.7&34.8 kDa |
| 表观分子量 | 19-21&40-60 kDa |
| 标签 | C-6His |
| 生物活性 | Measured by its ability to induce STAT reporter activity in 293F human embryonic kidney cells. The ED50 for this effect is 70-210 ng/ml. |
产品特性
| 纯度 | >95% as determined by reducing SDS-PAGE. |
| 内毒素 | < 1.0 EU per µg as determined by LAL test. |
| 保存 | Lyophilized protein should be stored at -5~-20℃, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at -5~-20℃ for 3 months. |
| 运输 | Ambient temperature or ice pack. |
| 制剂 | Lyophilized from a 0.2 μm filtered solution of 20mM PB, 200mM Trehalose, 4% Mannitol, 50mM NaCl, 0.02% Tween80, pH7.5. |
| 复融 | Always centrifuge tubes before opening. Do not mix by vortex or pipetting.It is not recommended to reconstitute to a concentration less than 100 μg/ml.Dissolve the lyophilized protein in distilled water.Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
背景介绍
Interleukin 23 (IL-23) is a heterodimeric Cytokine composed of two disulfide-linked subunits, a p19 subunit that is unique to IL-23, and a p40 subunit that is shared with IL-12. The p19 subunit has homology to the p35 subunit of IL-12, as well as to other single chain Cytokines such as IL-6 and IL-11. The p40 subunit is homologous to the extracellular domains of the hematopoietic Cytokine Receptors. Although p19 is expressed by activated macrophages, dendritic cells, T cells, and endothelial cells, only activated macrophages and dendritic cells express p40 concurrently to produce IL-23. IL-23 has biological activities that are similar to, but distinct from IL-12. Both IL-12 and IL-23 induce proliferation and IFN-gamma production by human T cells. While IL-12 acts on both naive and memory human T cells, the effects of IL-23 is restricted to memory T cells.
