产品信息
| 别名 | Fractalkine; C-X3-C Motif Chemokine 1; CX3C membrane-anchored Chemokine; Neurotactin; Small-inducible Cytokine D1; Cx3c; Fkn; Scyd1; CXC3; CXC3C; ABCD-3; SCYD1; C3Xkine; NTN; NTT |
| 物种 | Mouse |
| 表达宿主 | Human Cells |
| 序列信息 | Gln25-Arg337 |
| 检索号 | O35188 |
| 分子量 | 34.3 kDa |
| 表观分子量 | 50-65 kDa |
| 标签 | C-6His |
产品特性
| 纯度 | >95% as determined by reducing SDS-PAGE. |
| 内毒素 | < 1.0 EU per µg as determined by LAL test. |
| 保存 | Lyophilized protein should be stored at -5~-20℃, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at -5~-20℃ for 3 months. |
| 运输 | Ambient temperature or ice pack. |
| 制剂 | Lyophilized from a 0.2 μm filtered solution of 20mM PB,150mM NaCl,pH7.4. |
| 复融 | Always centrifuge tubes before opening. Do not mix by vortex or pipetting.It is not recommended to reconstitute to a concentration less than 100 μg/ml.Dissolve the lyophilized protein in distilled water.Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
背景介绍
Fractalkine (CX3CL1) is a single-pass type I membrane Protein and belongs to the intercrine delta family. It consists of an extracellular NH2-terminal domain, a mucin-like stalk, a transmembrane α helix, and a short cytoplasmic tail. CX3CL1 exists in two forms: as a membrane-anchored or as a shed 80-95K glyco Protein. Soluble CX3CL1 is generated by limited proteolysis on the cell surface, and a disintegrin and metallopeptidase 10 (ADAM10) and ADAM17/ tumor necrosis factor-α-converting enzyme (ADAM17/ TACE) participate in this shedding. It has been suggested that ADAM10 acts in the constitutive shedding, and ADAM17 acts in response to cell activation. The Protein may play a role in regulating leukocyte adhesion and migration processes at the endothelium.
