产品信息
| 别名 | Interleukin-1 Receptor-like 1; Lymphocyte antigen 84; Protein ST2; Protein T1; Il1rl1; DER4; Fit-1; IL-1 R4; IL-1RL1; IL-33R; Ly84; ST2L; ST2V; suppression of tumorigenicity 2 |
| 物种 | Mouse |
| 表达宿主 | Human Cells |
| 序列信息 | Ser27-Ala337 |
| 检索号 | P14719-2 |
| 分子量 | 36.6 kDa |
| 表观分子量 | 55-70 kDa |
| 标签 | C-6His |
| 生物活性 | Immobilized Mouse ST2-His at 5μg/ml (100 μl/well) can bind Rhesus Macaque IL-33-His.The ED50 of Rhesus Macaque IL-33-His is 82.03 ng/ml. |
产品特性
| 纯度 | >95% as determined by reducing SDS-PAGE. |
| 内毒素 | < 1.0 EU per µg as determined by LAL test. |
| 保存 | Lyophilized protein should be stored at -5~-20℃, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at -5~-20℃ for 3 months. |
| 运输 | Ambient temperature or ice pack. |
| 制剂 | Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. |
| 复融 | Always centrifuge tubes before opening. Do not mix by vortex or pipetting.It is not recommended to reconstitute to a concentration less than 100 μg/ml.Dissolve the lyophilized protein in distilled water.Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
背景介绍
ST2, also called IL-1 R4, is an Interleukin-1 Receptor family glyco Protein that plays a role in Th2 immune responses. ST2 is expressed on the surface of mast cells, activated Th2 cells, macrophages, and cardiac myocytes. This Receptor is very similar to the IL-1 Receptor type I and the IL-18 Receptor α chain in that ST2 also has three extracellular Ig domains and an intracellular Toll domain. ST2 binds IL-33, enhances inflammatory Cytokines by activating nuclear factor-κB (NF-κB) and mitogen activated Protein (MAP) kinases. ST2 exists as either a membrane bound form (ST2L) or as a soluble form (sST2). ST2L acts as a transmembrane signalling Receptor for IL-33 by mediating the effect of IL-33 on the inflammatory process, while sST2 can suppress IL-33 activity.
