产品信息
| 别名 | Dihydrolipoyl dehydrogenase (DLD); also called Dihydrolipoamide dehydrogenase; Glycine cleavage system L Protein; GCSL; LAD and PHE3; |
| 物种 | Mouse |
| 表达宿主 | Human Cells |
| 序列信息 | Ile26-Arg233 |
| 检索号 | P16382 |
| 分子量 | 51.5 kDa |
| 表观分子量 | 65-80 kDa |
| 生物活性 | Immobilized Mouse IL-4RA-Fc at 2μg/ml (100 μl/well)can bind Mouse IL-4-His.The ED50 of Mouse IL-4-His is 4.32 ng/ml. |
产品特性
| 纯度 | >95% as determined by reducing SDS-PAGE. |
| 内毒素 | < 1.0 EU per µg as determined by LAL test. |
| 保存 | Lyophilized protein should be stored at -5~-20℃, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at -5~-20℃ for 3 months. |
| 运输 | Ambient temperature or ice pack. |
| 制剂 | Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. |
| 复融 | Always centrifuge tubes before opening. Do not mix by vortex or pipetting.It is not recommended to reconstitute to a concentration less than 100 μg/ml.Dissolve the lyophilized protein in distilled water.Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
背景介绍
is a member of the class-I pyridine nucleotide-disulfide oxidoreductase family. Lipoamide dehydrogenase is a component of the glycine cleavage system as well as of the alpha-ketoacid dehydrogenase complexes. DLD exists as a homodimer. DLD is involved in the hyperactivation of spermatazoa during capacitation and in the spermatazoal acrosome reaction. Mutations in its encoding gene have been identified in patients with E3-deficient maple syrup urine disease and lipoamide dehydrogenase deficiency.
