产品信息
| 别名 | Interleukin-13; IL-13; T-Cell Activation Protein P600; Il13; Il-13 |
| 物种 | Mouse |
| 表达宿主 | Human Cells |
| 序列信息 | Ser26-Phe131 |
| 检索号 | P20109 |
| 分子量 | 12.7 kDa |
| 表观分子量 | 15-30 kDa |
| 标签 | C-6His |
| 生物活性 | Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. The ED50 for this effect is 2-13 ng/ml. |
产品特性
| 纯度 | >95% as determined by reducing SDS-PAGE. |
| 内毒素 | <1.0 EU per µg as determined by LAL test. |
| 保存 | Lyophilized protein should be stored at -5~-20℃, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at -5~-20℃ for 3 months. |
| 运输 | Ambient temperature or ice pack. |
| 制剂 | Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. |
| 复融 | Always centrifuge tubes before opening. Do not mix by vortex or pipetting.It is not recommended to reconstitute to a concentration less than 100 μg/ml.Dissolve the lyophilized protein in distilled water.Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
背景介绍
Mouse Interleukin 13 (mIL-13) is a pleiotropic Cytokine produced by activated Th2 cells. IL-13 induces B cell proliferation and immunoglobin production. It contains a four helical bundle with two internal disulfide bonds. Mouse IL13 shares 58% sequence identity with human Protein and exhibits cross-species activity. IL13 signals via Receptor IL13R (type2, IL4R) and activates STAT-6. IL13 initially binds IL-13Rα1 with low affinity and triggers association of IL4Rα, generating a high affinity heterodimeric Receptor IL13R and eliciting downstream signals. IL13 also binds IL-13Rα2 with high affinity, which plays a role in a negative feedback system of IL13 signaling. IL13 is an important mediator of allergic inflammation and disease.
