产品概述
检测原理 | Annexin V是一种钙离子依赖性磷脂结合蛋白,与磷脂酰丝氨酸PS有高度亲和力,可通过细胞外侧暴露的PS与凋亡早期细胞胞膜结合,将Annexin V标记荧光染料,利用流式细胞仪检测细胞凋亡。 细胞核染料(PI,DAPI,7-AAD)可与双链DNA特异性结合,并产生强烈的荧光,正常情况下无法透过细胞膜。由于凋亡晚期或坏死细胞膜丧失完整性,细胞核染料(DAPI、PI、7-AAD)可进入细胞内对DNA进行染色,与Annexin V搭配使用,可区分处于不同凋亡时期的细胞。 |
应用类型 | 细胞凋亡检测;Annexin V |
检测方法 | 荧光 |
样本类型 | 细胞 |
检测时间 | 40 分钟 |
检测仪器 | 流式细胞仪 |
荧光 | FITC |
激发/发射波长(EX/Em(nm)) | 490/530 |
流式仪通道设置 | FITC |
自备试剂 | PBS(PB180327), 去离子水 |
运输条件 | 冰袋 |
保藏温度 | 2~8℃,避光保存 |
产品组分
参考文献
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