产品信息
名称 | SU-DHL-4 (人B淋巴瘤细胞) (STR鉴定正确) |
别称 | SUDHL4; Sudhl4; SUDHL-4; Sudhl-4; SuDHL 4; SUD-4; SUD4; SU4; Stanford University-Diffuse Histiocytic Lymphoma-4; DHL-4; DHL4 |
种属 | 人类 |
生长特性 | 悬浮细胞 |
细胞形态 | 淋巴母细胞样 |
冻存条件 | 冻存液:55% 基础培养基+40%FBS+5%DMSO 温度:液氮 |
培养方案A(默认) |
培养条件:
气相:空气,95%;CO2,5%, 温度:37℃
|
注意事项 | 该细胞为悬浮细胞,请注意离心收集细胞悬液;请勿直接倒掉细胞培养液。 |
推荐传代比例 | 5×10^5-1×10^6cells/mL |
推荐换液频率 | 2-3次/周 |
注意事项 | 该细胞为悬浮细胞,请注意离心收集细胞悬液;请勿直接倒掉细胞培养液。 |
参考资料(来源文献)
背景描述 | Established from the peritoneal effusion of a 38-year-old man with B-NHL (diffuse large cell, cleaved cell type; originally described as "diffuse histiocytic lymphoma") in 1975; cell line carries EZH2 Y641S mutation; assigned to GCB-like lymphoma subtype (germinal center B-cell). Exome and RNA sequence data are available (see Ref 18187 and Exome sequence and RNA-Seq) |
年龄(性别) | 男性;38岁 |
组织来源 | 腹腔积液 |
细胞类型 | 肿瘤细胞 |
肿瘤类型 | 淋巴瘤细胞 |
倍增时间 | ~40 hours |
基因表达情况 | IgG+; Kappa+; IgM-; IgA-; IgD-; Lambda-; This cell line has relatively high expression levels of Bax; Bak; AIF; high caspase-9 activity. |
保藏机构 | ATCC; CRL-2957 DSMZ; ACC-495 |
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