产品信息
名称 | M-07e (人巨细胞白血病细胞) (STR鉴定正确) |
别称 | M-07E; M-O7e; M07-e; M07e; Mo7e; MO7e; M07E; MO7E |
种属 | 人类 |
生长特性 | 悬浮细胞 |
细胞形态 | 淋巴母细胞样 |
冻存条件 | 冻存液:90%FBS+10%DMSO 温度:液氮 |
培养方案A(默认) |
培养条件:
气相:空气,95%;CO2,5%, 温度:37℃
|
注意事项 | 该细胞为悬浮细胞,请注意离心收集细胞悬液;请勿直接倒掉细胞培养液。 |
推荐传代比例 | 5×10^5-1×10^6cells/mL |
推荐换液频率 | 2-3次/周 |
注意事项 | 该细胞为悬浮细胞,请注意离心收集细胞悬液;请勿直接倒掉细胞培养液。 |
参考资料(来源文献)
背景描述 | Established from the peripheral blood of a 6-month-old girl with acute megakaryoblastic leukemia (AML M7) at diagnosis in 1987; subline of the growth factor-independent cell line M-07; M-07e cells respond proliferatively to GM-CSF, IFN-alpha, IFN-beta, IFN-gamma, IL-2, IL-3, IL-4, IL-6, IL-15, NGF, SCF, TNF-alpha, TPO; we have noted that the cell line can become very quickly cytokine-independent (within 3-4 weeks), presumably due to outgrowth of independent cells. Exome and RNA sequence data are available |
年龄(性别) | 女性;6月龄 |
组织来源 | 外周血 |
细胞类型 | 肿瘤细胞 |
肿瘤类型 | 白血病细胞 |
生物安全等级 | BSL-1 |
倍增时间 | ~40 hours |
抗原表达情况 | CD3 -, CD13 +, CD14 -, CD19 -, CD33 +, HLA-DR - |
保藏机构 | DSMZ; ACC-104 |
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