HBE135-E6E7 (人支气管上皮细胞) (STR鉴定正确)
货号:CL-0732
规格:1×10⁶cells/T25培养瓶
价格: ¥1800
生长培养基:HBE135-E6E7 细胞专用培养基
产品信息
名称 | HBE135-E6E7 (人支气管上皮细胞) (STR鉴定正确) |
别称 | HBE 135-E6E7; HBE 135-E6/E7 |
种属 | 人 |
生长特性 | 贴壁细胞 |
细胞形态 | 上皮细胞样;卵圆形 |
冻存条件 | 冻存液:55%DM/F12+40%FBS+5%DMSO 温度:液氮 |
培养方案A(默认) |
生长培养基:
培养条件:
气相:空气,95%;CO2,5%, 温度:37℃
|
推荐传代比例 | 1:3-1:4 |
推荐换液频率 | 2-3次/周 |
参考资料(来源文献)
背景描述 | The HBE135-E6E7 cell line was derived from normal bronchial epithelium taken from a man undergoing lobectomy for squamous cell carcinoma. Cells from the primary explant in their first passage were infected with the recombinant retrovirus LXSN16E6E7 containing the human papilloma virus (HPV) E6E7 gene. Cells were selected in the presence of 0.4 mg/mL G418 |
年龄(性别) | 男性,;54岁 |
组织来源 | 肺;支气管 |
细胞类型 | 转化细胞系 |
生物安全等级 | BSL-2 |
倍增时间 | 24-30 hours |
保藏机构 | ATCC; CRL-2741 |
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