产品信息
| 名称 | DMS 53 (人肺癌细胞) (STR鉴定正确) |
| 别称 | DMS-53; DMS53; Darmouth Medical School 53 |
| 种属 | 人 |
| 生长特性 | 贴壁细胞 |
| 细胞形态 | 上皮细胞样 |
| 冻存条件 | 冻存液：55% 基础培养基+40%FBS+5%DMSO 温度：液氮 |
| 培养方案A（默认） |
生长培养基:
培养条件:
气相：空气，95%；CO2，5%, 温度：37℃
|
| 推荐传代比例 | 1:2-1:3 |
| 推荐换液频率 | 2-3次/周 |
参考资料(来源文献)
| 背景描述 | DMS 53 is a cell line exhibiting epithelial morphology that was isolated in 1974 from the lung of a 54-year-old, White male patient with carcinoma. |
| 年龄（性别） | 男性；54岁 |
| 组织来源 | 肺 |
| 细胞类型 | 肿瘤细胞 |
| 肿瘤类型 | 肺癌细胞 |
| 生物安全等级 | BSL-1 |
| 倍增时间 | 5.7 days (PubMed=6266631); 4.9 days (PubMed=2986244) |
| 致瘤性 | Yes; Yes, tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 10^7 cells. |
| 受体表达情况 | Bombesin, expressed; epidermal growth factor (EGF), expressed; transforming growth factor beta (TGF beta), expressed; acetylcholine, expressed |
| 抗原表达情况 | Leu 7; My23 |
| 基因表达情况 | adrenocorticotropin (adrenocorticotropic hormone, ACTH); bombesin; calcitonin; human chorionic gonadotropin (hCG); glucagon; growth hormone; 17 beta estradiol; thyroid releasing hormone; oxytocin - neurophysin (OT-NP); parathormone; somatostatin-like immunoreactivity (SRIF) |
| 保藏机构 | AddexBio; C0016027/4988 ATCC; CRL-2062 ECACC; 95062823 |
