产品信息
| 名称 | Panc 08.13 (人胰腺癌细胞) (STR鉴定正确) |
| 别称 | Panc 8.13; Panc-08.13; PANC-08-13; Panc_08_13; Panc08.13; Panc8.13; Panc-8_13; Panc-813; PANC 813; Panc813; PANC813; PANC0813; Panc0813; Pa14C; PL9; PL-9 |
| 种属 | 人 |
| 生长特性 | 贴壁细胞 |
| 细胞形态 | 上皮细胞样 |
| 冻存条件 | 冻存液：55% 基础培养基+40%FBS+5%DMSO 温度：液氮 |
| 培养方案A（默认） |
生长培养基:
培养条件:
气相：空气，95%；CO2，5%, 温度：37℃
|
| 推荐传代比例 | 1:2-1:4 |
| 推荐换液频率 | 2-3次/周 |
参考资料(来源文献)
| 背景描述 | Panc 08.13 is an epithelial cell line that was isolated in 1995 from the pancreas of a White, 85-year-old, male patient with adenocarcinoma. This cell line was deposited by EM Jaffee and can be used in cancer research.The cell line exhibits a K-ras oncogene mutation at codon 12 where a GGT --> GAT mutation resulted in substitution of aspartic acid for glycine. The cells have a reported plating efficiency of 40%. |
| 年龄（性别） | 男性；85岁 |
| 组织来源 | 胰腺 |
| 细胞类型 | 肿瘤细胞 |
| 肿瘤类型 | 胰腺癌细胞 |
| 生物安全等级 | BSL-1 |
| 倍增时间 | 20.0 hours (PubMed=9612602); 117 hours (PubMed=25984343) |
| 致瘤性 | Yes; Yes, forms tumors in nude or SCID mice |
| 抗原表达情况 | MHC class I +; MHC class II - |
| 基因表达情况 | cytokeratin 7; cytokeratin 18 |
| 保藏机构 | ATCC; CRL-2551 |
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