产品信息
| 别名 | B-TCGF, CSIF, TGIF |
| 物种 | Human |
| 表达宿主 | E.coli |
| 序列信息 | MSPGQGTQSENSCTHFPGNLPNMLRDLRDAFSRVKTFFQMKDQLDNLLLKESLLEDFKGYLGCQALSEMIQFYLEEVMPQAENQDPDIKAHVNSLGENLKTLRLRLRRCHRFLPCENKSKAVEQVKNAFNKLQEKGIYKAMSEFDIFINYIEAYMTMKIRN with polyhistidine tag at the C-terminus |
| 检索号 | P22301.1 |
| 分子量 | 19.6 kDa |
| 表观分子量 | 20 kDa |
| 标签 | His-tag at the C-terminus |
| 生物活性 | Measure by its ability to induce MC/9‑2 cells proliferation.The ED50 for this effect is <1 ng/mL.The specific activity of recombinant human IL-10 is approximately >1x10^6 IU/ mg. |
产品特性
| 纯度 | >98% as determined by SDS-PAGE. Ni-NTA chromatography |
| 内毒素 | <0.01 EU per 1 μg of the protein by the LAL method. |
| 保存 | Lyophilized protein should be stored at -5~-20°C for 1 year. Upon reconstitution, store at 2-8°C for up to 1 week. Further dilute in a buffer containing a carrier protein or stabilizer (e.g.0.1%BSA,10%FBS,5%HSA or 5% trehalose solution), protein aliquots should be stored at -5~-20°C or -80°C for 3-6 months. |
| 运输 | Ambient temperature or ice pack. |
| 制剂 | The protein was lyophilized from a 0.2 µm filtered solution containing 1X PBS, pH 8.0. |
| 复融 | It is recommended to reconstitute the lyophilized protein in sterile water to a concentration not less than 100 μg/mL.Do Not Vortex! Vigorous shaking may impair the biological activity of the protein. |
背景介绍
Interleukin 10 (IL-10), also known as human cytokine synthesis inhibitory factor (CSIF), is an anti-inflammatory cytokine. In humans, interleukin 10 is encoded by the IL10 gene. IL-10 signals through a receptor complex consisting of two IL-10 receptor-1 and two IL-10 receptor-2 proteins. Consequently, the functional receptor consists of four IL-10 receptor molecules. IL-10 binding induces STAT3 signalling via the phosphorylation of the cytoplasmic tails of IL-10 receptor 1 + IL-10 receptor 2 by JAK1 and Tyk2 respectively.
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