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R-spondin 1/RSPO1, Human, Recombinant

货号:PCK214

价格:¥1380¥3680¥1748025680

规格:
10μg
  • 10μg
  • 50μg
  • 500μg
  • 1mg
数量: - +

产品信息

别名 RSPO1, Roof plate-specific spondin-1
物种 Human
表达宿主 HEK293
序列信息 A DNA sequence encoding Human R-Spondin 1 Protein (#Q2MKA7) (Ser21 - Ala263)was expressed with polyhistidine-SUMO tag at the N-terminus.
检索号 Q2MKA7
分子量 42.43 kDa
表观分子量 68 kDa
标签 His-SUMO tag at the N-terminus
生物活性 R-Spondin-1 enhances BMP-2-mediated differentiation of MC3T3-E1 cells. The ED50 for this effect is 1-3 μg/mL.

产品特性

纯度 >95% as determined by SDS-PAGE. Ni-NTA chromatography
内毒素 <1 EU per 1 μg of the protein by the LAL method.
保存 Store at -5~-20°C or -80°C for 6 months. Further dilute in a buffer containing a carrier protein or stabilizer (e.g. 0.1% BSA, 10%FBS, 5%HSA or 5% trehalose solution), protein aliquots should be stored at -5~-20°C or -80°C for 3-6 months.
运输 The product is shipped on dry ice/polar packs.Upon receipt, store it immediately at the temperature listed below.
制剂 Liquid (1X PBS, pH 7.4)

背景介绍

R-spondin 1 (RSPO1, Roof plate-specific Spondin 1) is a cysteine-rich secreted glycoprotein that belongs to the R-Spondin family. Structurally, R-spondin 1 harbors an N-terminal signal peptide, two cysteine-rich furin-like (FU1-FU2) domains, a thrombospondin (TSP) domain, and a basic amino acid-rich (BR) C-terminal domain. R-spondin 1 acts as a ligand for the leucine-rich repeat-containing G-protein coupled receptor (LGR) family (LGR4/5/6), subsequent clearing negative regulators ZNRF3/RNF43 from the membrane, leading to the availability of Wnt receptor and activating Wnt/β-catenin signaling. The signal cascade is essential for regulating stem cell proliferation, embryonic development, and tissue homeostasis.
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