产品信息
| 别名 | RSPO1, Roof plate-specific spondin-1 |
| 物种 | Human |
| 表达宿主 | HEK293 |
| 序列信息 | A DNA sequence encoding Human R-Spondin 1 Protein (#Q2MKA7) (Ser21 - Ala263) was expressed with polyhistidine-SUMO tag at the N-terminus. |
| 检索号 | Q2MKA7 |
| 分子量 | 42.43 kDa |
| 表观分子量 | 68 kDa |
| 标签 | His-SUMO tag at the N-terminus |
| 生物活性 | R-Spondin-1 enhances BMP-2-mediated differentiation of MC3T3-E1 cells. The ED50 for this effect is 1-3 μg/mL. |
产品特性
| 纯度 | >95% as determined by SDS-PAGE. Ni-NTA chromatography |
| 内毒素 | <1 EU per 1 μg of the protein by the LAL method. |
| 保存 | Store at -5~-20°C or -80°C for 6 months. Further dilute in a buffer containing a carrier protein or stabilizer (e.g. 0.1% BSA, 10%FBS, 5%HSA or 5% trehalose solution), protein aliquots should be stored at -5~-20°C or -80°C for 3-6 months. |
| 运输 | The product is shipped on dry ice/polar packs. Upon receipt, store it immediately at the temperature listed below. |
| 制剂 | Liquid (1X PBS, pH 7.4) |
背景介绍
R-spondin 1 (RSPO1, Roof plate-specific Spondin 1) is a cysteine-rich secreted glycoprotein that belongs to the R-Spondin family. Structurally, R-spondin 1 harbors an N-terminal signal peptide, two cysteine-rich furin-like (FU1-FU2) domains, a thrombospondin (TSP) domain, and a basic amino acid-rich (BR) C-terminal domain. R-spondin 1 acts as a ligand for the leucine-rich repeat-containing G-protein coupled receptor (LGR) family (LGR4/5/6), subsequent clearing negative regulators ZNRF3/RNF43 from the membrane, leading to the availability of Wnt receptor and activating Wnt/β-catenin signaling. The signal cascade is essential for regulating stem cell proliferation, embryonic development, and tissue homeostasis.
