产品信息
| 别名 | Tumor necrosis factor Receptor superfamily member 1B; TNFRSF1B; Tumor necrosis factorReceptor 2; TNF-R2; TNF-RII; Tumor necrosis factor Receptor type II; p75; p80 TNF-alphaReceptor; CD120b |
| 物种 | Human |
| 表达宿主 | Human Cells |
| 序列信息 | Pro24-Thr206 |
| 检索号 | P20333 |
| 分子量 | 46.5 kDa |
| 表观分子量 | 60 kDa |
| 生物活性 | Immobilized Recombinant Human TNF RII-mFc at 1μg/ml (100 μl/well) can bind Recombinant Human TNF alpha-His. The ED50 of Recombinant Human TNF alpha-His is 1.97 ng/ml. |
产品特性
| 纯度 | >95% as determined by reducing SDS-PAGE. |
| 内毒素 | < 1.0 EU per µg as determined by LAL test. |
| 保存 | Lyophilized protein should be stored at -5~-20℃, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at -5~-20℃ for 3 months. |
| 运输 | Ambient temperature or ice pack. |
| 制剂 | Lyophilized from a 0.2 μm filtered solution of PBS,pH7.4. |
| 复融 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
背景介绍
Tumor necrosis factor Receptor superfamily member 1B (TNFRSF1B) is a member of the tumor necrosisfactor Receptor superfamily. Human TNF RII contains four cysteinerich repeats in its ECD, which shares 58%and 56% amino acid sequence identity with the mouse and rat orthologs, respectively.TNF RII is expressedpredominantly on cells of the hematopoietic lineage, such as T and natural killer cells, as well as onendothelial cells, microglia, astrocytes,neurons, oligodendrocytes, cardiac myocytes, thymocytes, andmesenchymal stem cells.TNF RII binds to the membranebound forms of TNFα and Lymphotoxinα/ TNFβ;soluble TNF is thought to signal predominately through TNF RI.Soluble TNF RII is believed to inhibit TNFbiological activity by binding TNF thereby preventing it from activating membrane TNF Receptors.
